1.Do not seal the containers.
Cryobiological storage containers are designed to function with little or no internal pressure. The use of any tight-fitting stopper or plug that prevents the adequate venting of gas builds up pressure that could severely damage or even burst the container.
2.Use only liquid nitrogen in liquid nitrogen container. Do not ever use liquid air or liquid oxygen in these containers. because either of which could present a combustion hazard.
3.Liquid nitrogen tank vacuum mouth, valve seal, seal cannot be damaged.
4.When used to should take put down gently,avoids colliding.
5.Never overfill the containers.
6.Use for the first time,The heat balance for a long time,that it can a small amount of liquid medium filling precooling,then slowly filled.